Streptozotocininduced diabetic models in mice and rats. Myricitrin ameliorates hyperglycemia, glucose intolerance. Streptozotocin is a glucosaminenitrosourea compound. In mice, the type 1 diabetes may be induced either by a single higher dose of streptozotocin or by repeated application of lower doses, both applied intraperitoneally. Injection of streptozotocin caused a threefold elevation of blood glucose by comparison with the control mice at four, six and eight weeks of diabetes. Rodent models of streptozotocininduced diabetic nephropathy. Thus we hypothesized that exogenous leptin administered to mice with streptozotocin stzinduced diabetes reverses hyperglycemia through direct action on. However, the effects of diabetes on genital tissue structure, innervation and function remains poorly characterized. The present study was designed to evaluate the protective effect of sulphurenic acid sa, a pure compound from antrodia camphorata, on diabetes and hyperlipidemia in an animal model study and to clarify the underlying molecular mechanism.
Characterisation of streptozotocin induced diabetes mellitus. Streptozotocin use in mice and rats version 1 date of publication. Stz diabetic rats were treated with oral doses of mebs 200 and 400 mgkg for 8 weeks. To study the protective effect of medicines on the formation of atherosclerosis in mice, it is needed to conduct the study in mice which is not genetically diabetic mice induced by streptozotocin stz to produce hyperglycemia and atherosclerosis, compared with. Table 1 items required for establishing stzinduced diabetes in rodents chemical reagents equipment consumables streptozotocin electronic weighing balance for streptozotocin 10. Evaluation of antidiabetic activity of euphorbia hirta linn. Chronic hyperglycemia and the corresponding glucotoxicity are the main pathogenic.
The dna sensor aim2 protects against streptozotocin. Stzinduced diabetes is characterized by pancreatic. Streptozotocin diabetes induced by multiple low doses of the. Leptin therapy reverses hyperglycemia in mice with streptozotocininduced diabetes, independent of hepatic leptin signaling heather c. Research design and methods five groups of micewildtype wt, nox2. Streptozotocin induces one type of diabetes which is similar to diabetes mellitus with nonketosis hyperglycemia in some. Insulin, a peptide hormone has been reported to have key functions in the brain and in alleviating several psychological impairments, occur as a consequence of diabetes. Diabetes is associated with declining sexual function in women. If mice in the stzinjected group attain a diabetic state by day 14, retest. Furthermore, streptozotocininduced diabetes caused a progressive loss of weight from 4 to 8 weeks and a. Alloxan diabetes and streptozotocin diabetes figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected 22. Kieffer 0 1 3 0 department of surgery, university of british columbia, van couver.
The streptozotocininduced diabetic nude mouse model ncbi nih. Apr 25, 2014 clinical and preclinical data suggest that diabetes is often associated with anxiety. The timing and appearance of the inflammatory islet lesions suggest but do not prove that streptozotocin acts by initiating a cellmediated immune reaction. Ijms free fulltext antidiabetic and antihyperlipidemic. In this study, diabetes was induced by a single injection of streptozotocin stz, 45 mgkg, i. The animal experiments were performed according to the guidelines for laboratory animals established by the wuhan university center for animal center experimenta3lab, and all study protocols were subjected to approval by the institutional center of wuhan university wuhan, china. The present study was designed to investigate the characteristics of diabetes mellitus induced by. The mechanisms of alloxan and streptozotocininduced diabetes.
Streptozotocin stzinduced diabetic animal models are useful. As with other alkylating agents in the nitrosourea class, it is toxic to cells by causing damage to the dna, though other mechanisms may also contribute. The responses are accompanied by corresponding inverse changes in plasma insulin and sequential ultrastructural changes resulting in necrotic beta cell death. Dna damage induces activation of parp which is likely more important for diabetes induction than the dna damage itself. Polyadpribose polymerasedeficient mice are protected from. Diabetes is induced in c57bl6j, nodscid, and nsg male mice using validated protocols.
Partial protective effect of rutin on multiple low dose. Sep 01, 2015 streptozotocin stz is an antibiotic that produces pancreatic islet. Oxidative stress is one of the major causes of betacell death. In this study, sharply reduced bodyweights and increased fasting blood glucose levels were observed in mice with stzinduced diabetes, and both were significantly reversed by administration of ios.
Pdf characterisation of streptozotocin induced diabetes. The present study aimed to evaluate the antidiabetic effects of apigenin in mice with streptozotocin stzinduced diabetes. Streptozotocin stz is an antibiotic that produces pancreatic islet. Type 1 diabetes t1d is a pathological condition characterized by the loss of insulinproducing. Antidiabetic effect of betulinic acid on streptozotocin.
Oral administration of pcs extract resulted in a significant improvement of hyperglycemia in streptozotocin induced diabetic mice. Ameliorative potential of morin in streptozotocininduced neuropathic pain in rats. The present study aimed to evaluate the antidiabetic effects of apigenin in mice with streptozotocin stz induced diabetes. Streptozotocin stz induced diabetes mellitus dm offers a very cost effective and expeditious technique that can be used in most strains of rodents, opening the field of dm research to an array of genotypic and phenotypic options that would otherwise be inaccessible.
Research design and methods five groups of micewild. Streptozotocininduced experimental diabetes in male wistar. Streptozotocininduced type 1 diabetes in rodents as a model. Nox2 deficiency protects against streptozotocininduced.
Metabolic and vascular alterations induced by streptozotocin induced diabetes. Characterisation of pain responses in the high fat dietstreptozotocin model of diabetes and the analgesic effects of antidiabetic treatments. Swiss albino mice of either sex were intraperitoneally administered single dose of streptozotocin 180 mgkg, or 100 mgkg and multiple low dose of streptozotocin 40 mgkgday. We evaluated nude male mice from various sources for their sensitivity to a single high dose 160 to 240 mgkg of stz. Effects of stzinduced diabetes on the relative weights of. Characterisation of pain responses in the high fat diet streptozotocin model of diabetes and the analgesic effects of antidiabetic treatments.
Cardiac histopathology was evaluated by hematoxylin and eosin, sirius red, and immuno. High cell mass prevents streptozotocininduced diabetes in thioredoxininteracting proteinde. Oral administration of pcs extract resulted in a significant improvement of hyperglycemia in streptozotocininduced diabetic mice. Characterisation of streptozotocin induced diabetes mellitus in swiss albino mice. Insulin hypersensitivity and resistance to streptozotocininduced diabetes in mice lacking pten in adipose tissue. In control and streptozotocintreated female rats, we investigated the effects of diabetes on vaginal blood flow, tissue morphology, and expression of arginase i, endothelial nitric oxide synthase enos and cgmp. Streptozotocin stz induced diabetes in mice and rats has been used widely as an animal model to study type i. Exposure of fluoride with streptozotocininduced diabetes. C57bl6 mice were injected with streptozotocin to induce diabetes. The epidemic of type 2 diabetes mellitus t2dm is fueled by added fructose consumption. Therefore, this study was designed to evaluate the antidiabetic potential of black ginseng extract bge in streptozotocin stzinduced insulindeficient diabetic mice, in. Figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected.
Treatment with streptozotocin increases blood glucose levels in mice by itself before the septic challenge figure 1a. Black ginseng extract counteracts streptozotocininduced. Streptozotocininduced diabetic neuropathy in mice and rats. The experimental and control groups were treated with 5 lg of tlr4 small interfering rna sirna or scrambled sirna. Leptin therapy reverses hyperglycemia in mice with. Insulin hypersensitivity and resistance to streptozotocin induced diabetes in mice lacking pten in adipose tissue. Increasing atherosclerosis in streptozotocininduced diabetes.
Yet the mechanism of leptinmediated reversal of hyperglycemia has not been fully defined. Here, we thus combined highfathighfructose diet, with multiple lowdose injections of streptozotocin hf. Reduced sirtuin1 signalling exacerbates diabetic mice. However, its effects in diabetesinduced anxiety are scanty. Clinical and preclinical data suggest that diabetes is often associated with anxiety. The streptozotocininduced diabetic nude mouse model. Experimental design animals were fasted overnight and were divided in. Single dose streptozotocininduced diabetes laboratory animals.
Streptozotocin stzinduced diabetes in mice and rats has been used widely as an animal model to study type i. Full text streptozotocininduced type 1 diabetes in rodents. Combination of highfathighfructose diet and lowdose. Twelve days after streptozotocin injection, mice with blood glucose level 140mgdl were separated and used for the study. As blood glucose levels are considered an accurate diagnostic tool for diabetes, there.
Objectives a complex endocrine and metabolic disorder, diabetes mellitus affects about 6% of the population worldwide and is rapidly reaching epidemic proportions. Streptozotocininduced diabetes in the rat is associated with. Detailed in this unit are protocols for producing stzinduced insulin deficiency and hyperglycemia in mice and rats. Full text streptozotocininduced type 1 diabetes in rodents as a. The liver is a key organ regulating glucose metabolism and is also a target of leptin action. Diabetes was induced in mice by intraperitoneal injection of streptozotocin 50 mgkg after. Diabetes was induced with streptozotocin, the standard and most common method for experimental diabetes 35, 36,41. Streptozotocininduced type 1 diabetes in rodents as a model for studying mitochondrial mechanisms of diabetic.
Therefore, this study was designed to evaluate the antidiabetic potential of black ginseng extract bge in streptozotocin stz induced insulindeficient diabetic mice, in comparison with red ginseng extract rge. To test the hypothesis that myricitrin myr improves type 2 diabetes, we examined the e ect of myr on hyperglycemia, glucose intolerance, hepatic steatosis, and in. We hypothesized that nox2 deficiency decreases reactive oxygen species ros production and immune response and protects against streptozotocin stzinduced. Diabetes is induced in mice by using streptozotocin stz, a compound that has a preferential toxicity toward pancreatic. Streptozotocininduced diabetic ratsmice stz model advantages. Research article exposure of fluoride with streptozotocininduced diabetes aggravates testicular damage and spermatozoa parameters in mice manuel sanchezgutierrez,1 evelia martinezloredo,1. Ultrastructural evidence of abundant type c viruses within beta cells of. Type 1 mlds diabetes was induced in adult male swiss albino mice 2830 g by multiple intraperitoneal i. The present study was designed to investigate the characteristics of diabetes mellitus induced by different doses of streptozotocin in mice as assessed by blood glucose concentration. Dec 18, 2007 figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected. Antidiabetic activities of polysaccharides separated from.
Streptozotocin induced diabetes mice were made diabetic by a single intraperitoneal injection of streptozotocin 150 mgkg i. Leptin therapy reverses hyperglycemia in mice with streptozotocin induced diabetes, independent of hepatic leptin signaling heather c. Stz induced diabetes represents an immunemediated mouse model of t1d. Polyadpribose polymerasedeficient mice are protected. Pdf preventative effects of resveratrol and estradiol on. Thus we hypothesized that exogenous leptin administered to mice with streptozotocin stz induced diabetes reverses hyperglycemia through direct action on.
Because 8% to 12% of diabetic pregnant women present with malformed fetuses, there is great interest in understanding the etiology, pathophysiological mechanisms, and treatment of gestational diabetes. George fantus department of medicine, mount sinai hospital, toronto general research institute, banting and best diabetes. Pdf the objective of this study is to induce experimental diabetes mellitus by streptozotocin in normal adult wistar rats via comparison of. Biomimetic carbon monoxide nanogenerator ameliorates. Ameliorative potential of morin in streptozotocin induced neuropathic pain in rats. An inventory of c57bl6j males expedites access to diabetic mice. The results showed that the expected rise in blood glucose levels induced by stz treatment preceded the surge in corticosterone secretion, which took place 1 d after diabetes. Sigma chemicals, mo, usa 40 mgkg, body weight once daily for 5. Insulin hypersensitivity and resistance to streptozotocin. Systemic delivery of sirna specific for silencing tlr4.
Pdf induction of diabetes by streptozotocin in rats researchgate. Glucose homeostasis is controlled by endocrine pancreatic cells, and any pancreatic disturbance can result in diabetes. In our study, the lxr agonist t09017 protected against hli injury in diabetic mice by attenuating endothelial os and stimulating angiogenesis. Opposite effects of angiotensins receptors type 2 and type 4. Objective the role of nox2containing nadph oxidase in the development of diabetes is not fully understood. It was thus, interesting to see the role of wdr in streptozotocinmediated diabetes in mice, a model for type i diabetes. In case of single dose application, a reliable induction is described for doses of 200 mgkg anderson et al. The rat mouse model of diabetes mellitus induced by streptozotocin injections is one of the most widely used model of type i diabetes and mimics human pathological situation of decreased insulin production untreated type i diabetes or decrease tissue responsiveness to insulin. Streptozotocin experimental diabetes male wistar rats introduction streptozotocininduced diabetes in laboratory animals presents an experimental model whose value is in the elucidation of causal relationships related to human diabetes mellitus. Islets from mldstreated ripm3 mice had fewer inflammatory cells and. Metabolic and vascular alterations induced by streptozotocininduced diabetes.
In this study, we investigated the effects of psoralea corylifolia l. Furthermore, streptozotocin induced diabetes caused a progressive loss of weight from 4 to 8 weeks and a. To study the protective effect of medicines on the formation of atherosclerosis in mice, it is needed to conduct the study in mice which is not genetically diabetic mice induced by streptozotocin stz to produce hyperglycemia and atherosclerosis, compared with mice treated by yolk or its combination. Diabetes was induced by daily 55 mgkg intraperitoneal injections of streptozotocin stz solution over five days. Adrenal hypersensitivity precedes chronic hypercorticism in. To test this hypothesis, we used the streptozotocin stzinduced diabetes mouse model and measured hypothalamicpituitaryadrenal axis activity at different time points. The effect of streptozotocininduced diabetes in rats. Effects of stzinduced diabetes on the relative weights of kidney, liver and pancreas in albino rats. Methods diabetes in mice was induced by intraperitoneal injection of a single dose of stz 200 mgkg body. In control and streptozotocin treated female rats, we investigated the effects of diabetes on vaginal blood flow, tissue morphology, and expression of arginase i, endothelial nitric oxide synthase enos and cgmp. Pdf characterization of streptozotocin induced diabetes. However, its effects in diabetes induced anxiety are scanty.
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